A Genome-Scale Metabolic Reconstruction of Phytophthora infestans With the Integration of Transcriptional Data Reveals the Key Metabolic Patterns Involved in the Interaction of Its Host.
Identifieur interne : 000877 ( Main/Exploration ); précédent : 000876; suivant : 000878A Genome-Scale Metabolic Reconstruction of Phytophthora infestans With the Integration of Transcriptional Data Reveals the Key Metabolic Patterns Involved in the Interaction of Its Host.
Auteurs : David Botero [Colombie] ; Iván Valdés [Colombie] ; María-Juliana Rodríguez [Colombie] ; Diana Henao [Colombie] ; Giovanna Danies [Colombie] ; Andrés F. González [Colombie] ; Silvia Restrepo [Colombie]Source :
- Frontiers in genetics [ 1664-8021 ] ; 2018.
Abstract
Phytophthora infestans, the causal agent of late blight disease, affects potatoes and tomatoes worldwide. This plant pathogen has a hemibiotrophic lifestyle, having an initial biotrophic infection phase during which the pathogen spreads within the host tissue, followed by a necrotrophic phase in which host cell death is induced. Although increasing information is available on the molecular mechanisms, underlying the distinct phases of the hemibiotrophic lifestyle, studies that consider the entire metabolic processes in the pathogen while undergoing the biotrophic, transition to necrotrophic, and necrotrophic phases have not been conducted. In this study, the genome-scale metabolic reconstruction of P. infestans was achieved. Subsequently, transcriptional data (microarrays, RNA-seq) was integrated into the metabolic reconstruction to obtain context-specific (metabolic) models (CSMs) of the infection process, using constraint-based reconstruction and analysis. The goal was to identify specific metabolic markers for distinct stages of the pathogen's life cycle. Results indicate that the overall metabolism show significant changes during infection. The most significant changes in metabolism were observed at the latest time points of infection. Metabolic activity associated with purine, pyrimidine, fatty acid, fructose and mannose, arginine, glycine, serine, and threonine amino acids appeared to be the most important metabolisms of the pathogen during the course of the infection, showing high number of reactions associated with them and expression switches at important stages of the life cycle. This study provides a framework for future throughput studies of the metabolic changes during the hemibiotrophic life cycle of this important plant pathogen.
DOI: 10.3389/fgene.2018.00244
PubMed: 30042788
PubMed Central: PMC6048221
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Genome-Scale Metabolic Reconstruction of <i>Phytophthora infestans</i> With the Integration of Transcriptional Data Reveals the Key Metabolic Patterns Involved in the Interaction of Its Host.</title><author><name sortKey="Botero, David" sort="Botero, David" uniqKey="Botero D" first="David" last="Botero">David Botero</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Valdes, Ivan" sort="Valdes, Ivan" uniqKey="Valdes I" first="Iván" last="Valdés">Iván Valdés</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Rodriguez, Maria Juliana" sort="Rodriguez, Maria Juliana" uniqKey="Rodriguez M" first="María-Juliana" last="Rodríguez">María-Juliana Rodríguez</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Henao, Diana" sort="Henao, Diana" uniqKey="Henao D" first="Diana" last="Henao">Diana Henao</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Danies, Giovanna" sort="Danies, Giovanna" uniqKey="Danies G" first="Giovanna" last="Danies">Giovanna Danies</name><affiliation wicri:level="1"><nlm:affiliation>Department of Design, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Department of Design, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Gonzalez, Andres F" sort="Gonzalez, Andres F" uniqKey="Gonzalez A" first="Andrés F" last="González">Andrés F. González</name><affiliation wicri:level="1"><nlm:affiliation>Group of Product and Process Design, Department of Chemical Engineering, Universidad de Los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Group of Product and Process Design, Department of Chemical Engineering, Universidad de Los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Restrepo, Silvia" sort="Restrepo, Silvia" uniqKey="Restrepo S" first="Silvia" last="Restrepo">Silvia Restrepo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30042788</idno><idno type="pmid">30042788</idno><idno type="doi">10.3389/fgene.2018.00244</idno><idno type="pmc">PMC6048221</idno><idno type="wicri:Area/Main/Corpus">000710</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000710</idno><idno type="wicri:Area/Main/Curation">000710</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000710</idno><idno type="wicri:Area/Main/Exploration">000710</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Genome-Scale Metabolic Reconstruction of <i>Phytophthora infestans</i> With the Integration of Transcriptional Data Reveals the Key Metabolic Patterns Involved in the Interaction of Its Host.</title><author><name sortKey="Botero, David" sort="Botero, David" uniqKey="Botero D" first="David" last="Botero">David Botero</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Valdes, Ivan" sort="Valdes, Ivan" uniqKey="Valdes I" first="Iván" last="Valdés">Iván Valdés</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Rodriguez, Maria Juliana" sort="Rodriguez, Maria Juliana" uniqKey="Rodriguez M" first="María-Juliana" last="Rodríguez">María-Juliana Rodríguez</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Henao, Diana" sort="Henao, Diana" uniqKey="Henao D" first="Diana" last="Henao">Diana Henao</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Danies, Giovanna" sort="Danies, Giovanna" uniqKey="Danies G" first="Giovanna" last="Danies">Giovanna Danies</name><affiliation wicri:level="1"><nlm:affiliation>Department of Design, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Department of Design, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Gonzalez, Andres F" sort="Gonzalez, Andres F" uniqKey="Gonzalez A" first="Andrés F" last="González">Andrés F. González</name><affiliation wicri:level="1"><nlm:affiliation>Group of Product and Process Design, Department of Chemical Engineering, Universidad de Los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Group of Product and Process Design, Department of Chemical Engineering, Universidad de Los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Restrepo, Silvia" sort="Restrepo, Silvia" uniqKey="Restrepo S" first="Silvia" last="Restrepo">Silvia Restrepo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in genetics</title><idno type="ISSN">1664-8021</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Phytophthora infestans</i>, the causal agent of late blight disease, affects potatoes and tomatoes worldwide. This plant pathogen has a hemibiotrophic lifestyle, having an initial biotrophic infection phase during which the pathogen spreads within the host tissue, followed by a necrotrophic phase in which host cell death is induced. Although increasing information is available on the molecular mechanisms, underlying the distinct phases of the hemibiotrophic lifestyle, studies that consider the entire metabolic processes in the pathogen while undergoing the biotrophic, transition to necrotrophic, and necrotrophic phases have not been conducted. In this study, the genome-scale metabolic reconstruction of <i>P. infestans</i> was achieved. Subsequently, transcriptional data (microarrays, RNA-seq) was integrated into the metabolic reconstruction to obtain context-specific (metabolic) models (CSMs) of the infection process, using constraint-based reconstruction and analysis. The goal was to identify specific metabolic markers for distinct stages of the pathogen's life cycle. Results indicate that the overall metabolism show significant changes during infection. The most significant changes in metabolism were observed at the latest time points of infection. Metabolic activity associated with purine, pyrimidine, fatty acid, fructose and mannose, arginine, glycine, serine, and threonine amino acids appeared to be the most important metabolisms of the pathogen during the course of the infection, showing high number of reactions associated with them and expression switches at important stages of the life cycle. This study provides a framework for future throughput studies of the metabolic changes during the hemibiotrophic life cycle of this important plant pathogen.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30042788</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-8021</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Frontiers in genetics</Title><ISOAbbreviation>Front Genet</ISOAbbreviation></Journal><ArticleTitle>A Genome-Scale Metabolic Reconstruction of <i>Phytophthora infestans</i> With the Integration of Transcriptional Data Reveals the Key Metabolic Patterns Involved in the Interaction of Its Host.</ArticleTitle><Pagination><MedlinePgn>244</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fgene.2018.00244</ELocationID><Abstract><AbstractText><i>Phytophthora infestans</i>, the causal agent of late blight disease, affects potatoes and tomatoes worldwide. This plant pathogen has a hemibiotrophic lifestyle, having an initial biotrophic infection phase during which the pathogen spreads within the host tissue, followed by a necrotrophic phase in which host cell death is induced. Although increasing information is available on the molecular mechanisms, underlying the distinct phases of the hemibiotrophic lifestyle, studies that consider the entire metabolic processes in the pathogen while undergoing the biotrophic, transition to necrotrophic, and necrotrophic phases have not been conducted. In this study, the genome-scale metabolic reconstruction of <i>P. infestans</i> was achieved. Subsequently, transcriptional data (microarrays, RNA-seq) was integrated into the metabolic reconstruction to obtain context-specific (metabolic) models (CSMs) of the infection process, using constraint-based reconstruction and analysis. The goal was to identify specific metabolic markers for distinct stages of the pathogen's life cycle. Results indicate that the overall metabolism show significant changes during infection. The most significant changes in metabolism were observed at the latest time points of infection. Metabolic activity associated with purine, pyrimidine, fatty acid, fructose and mannose, arginine, glycine, serine, and threonine amino acids appeared to be the most important metabolisms of the pathogen during the course of the infection, showing high number of reactions associated with them and expression switches at important stages of the life cycle. This study provides a framework for future throughput studies of the metabolic changes during the hemibiotrophic life cycle of this important plant pathogen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Botero</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Valdés</LastName><ForeName>Iván</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodríguez</LastName><ForeName>María-Juliana</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henao</LastName><ForeName>Diana</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Danies</LastName><ForeName>Giovanna</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Design, Universidad de los Andes, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>González</LastName><ForeName>Andrés F</ForeName><Initials>AF</Initials><AffiliationInfo><Affiliation>Group of Product and Process Design, Department of Chemical Engineering, Universidad de Los Andes, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Restrepo</LastName><ForeName>Silvia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratory of Mycology and Phytopathology (LAMFU), Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>07</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Genet</MedlineTA><NlmUniqueID>101560621</NlmUniqueID><ISSNLinking>1664-8021</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">context-specific models</Keyword><Keyword MajorTopicYN="N">flux balance analysis</Keyword><Keyword MajorTopicYN="N">hemibiotrophy</Keyword><Keyword MajorTopicYN="N">metabolic reconstruction</Keyword><Keyword MajorTopicYN="N">plant-pathogen interaction</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>01</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>06</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>7</Month><Day>26</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30042788</ArticleId><ArticleId IdType="doi">10.3389/fgene.2018.00244</ArticleId><ArticleId IdType="pmc">PMC6048221</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>BMC Bioinformatics. 2010 Apr 29;11:213</Citation><ArticleIdList><ArticleId IdType="pubmed">20426874</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2009 Sep 17;461(7262):393-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19741609</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Biotechnol. 2010 Feb;21(1):70-7</Citation><ArticleIdList><ArticleId IdType="pubmed">20171871</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2004 Feb;5(2):101-13</Citation><ArticleIdList><ArticleId IdType="pubmed">14735121</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2010 May;62(3):357-66</Citation><ArticleIdList><ArticleId IdType="pubmed">20128886</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2011 Feb 15;27(4):541-7</Citation><ArticleIdList><ArticleId IdType="pubmed">21172910</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2009 Feb 1;102(2):583-97</Citation><ArticleIdList><ArticleId IdType="pubmed">18985611</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Nov 26;8(11):e81606</Citation><ArticleIdList><ArticleId IdType="pubmed">24303057</ArticleId></ArticleIdList></Reference><Reference><Citation>Brief Bioinform. 2014 Jan;15(1):108-22</Citation><ArticleIdList><ArticleId IdType="pubmed">23131418</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Microbiol. 2010 Jun;13(3):344-9</Citation><ArticleIdList><ArticleId IdType="pubmed">20430689</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2013;3:2398</Citation><ArticleIdList><ArticleId IdType="pubmed">23928947</ArticleId></ArticleIdList></Reference><Reference><Citation>ScientificWorldJournal. 2014 Feb 24;2014:416289</Citation><ArticleIdList><ArticleId IdType="pubmed">24707203</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2007 Aug;19(8):2349-69</Citation><ArticleIdList><ArticleId IdType="pubmed">17675403</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2010 Jun;5(6):769-72</Citation><ArticleIdList><ArticleId IdType="pubmed">20400849</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2014 Mar 10;537(2):312-21</Citation><ArticleIdList><ArticleId IdType="pubmed">24361203</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Syst Biol. 2013 Nov 19;9:708</Citation><ArticleIdList><ArticleId IdType="pubmed">24247825</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2007 Jan;35(Database issue):D274-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17135193</ArticleId></ArticleIdList></Reference><Reference><Citation>J Theor Biol. 2008 Jun 7;252(3):497-504</Citation><ArticleIdList><ArticleId IdType="pubmed">18249414</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2007 Jun 20;8:212</Citation><ArticleIdList><ArticleId IdType="pubmed">17584497</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2012;8(10):e1002940</Citation><ArticleIdList><ArticleId IdType="pubmed">23055926</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2011 Aug 04;6(9):1290-307</Citation><ArticleIdList><ArticleId IdType="pubmed">21886097</ArticleId></ArticleIdList></Reference><Reference><Citation>Metabolites. 2013 Jan 08;3(1):1-23</Citation><ArticleIdList><ArticleId IdType="pubmed">24957887</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2005 Mar;42(3):244-56</Citation><ArticleIdList><ArticleId IdType="pubmed">15707845</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2002 Jan 1;30(1):59-61</Citation><ArticleIdList><ArticleId IdType="pubmed">11752254</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Syst Biol. 2011 Sep 23;5:147</Citation><ArticleIdList><ArticleId IdType="pubmed">21943338</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2013 Jul 17;14:483</Citation><ArticleIdList><ArticleId IdType="pubmed">23865555</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2007 Jul;35(Web Server issue):W182-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17526522</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2008 May;9(3):385-402</Citation><ArticleIdList><ArticleId IdType="pubmed">18705878</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2010 Mar;28(3):245-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20212490</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2002;40:349-79</Citation><ArticleIdList><ArticleId IdType="pubmed">12147764</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2017 Feb 23;18(1):198</Citation><ArticleIdList><ArticleId IdType="pubmed">28228125</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Jul 09;10(7):e0131875</Citation><ArticleIdList><ArticleId IdType="pubmed">26158726</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2012 Mar;63(6):2343-51</Citation><ArticleIdList><ArticleId IdType="pubmed">22378949</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2018 Jun;19(6):1403-1413</Citation><ArticleIdList><ArticleId IdType="pubmed">28990716</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2009 Nov;10(6):843-55</Citation><ArticleIdList><ArticleId IdType="pubmed">19849790</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1999 Jan 1;27(1):29-34</Citation><ArticleIdList><ArticleId IdType="pubmed">9847135</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2010 Jan;5(1):93-121</Citation><ArticleIdList><ArticleId IdType="pubmed">20057383</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2015 Jan;43(Database issue):D204-12</Citation><ArticleIdList><ArticleId IdType="pubmed">25348405</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Colombie</li></country></list><tree><country name="Colombie"><noRegion><name sortKey="Botero, David" sort="Botero, David" uniqKey="Botero D" first="David" last="Botero">David Botero</name></noRegion><name sortKey="Danies, Giovanna" sort="Danies, Giovanna" uniqKey="Danies G" first="Giovanna" last="Danies">Giovanna Danies</name><name sortKey="Gonzalez, Andres F" sort="Gonzalez, Andres F" uniqKey="Gonzalez A" first="Andrés F" last="González">Andrés F. González</name><name sortKey="Henao, Diana" sort="Henao, Diana" uniqKey="Henao D" first="Diana" last="Henao">Diana Henao</name><name sortKey="Restrepo, Silvia" sort="Restrepo, Silvia" uniqKey="Restrepo S" first="Silvia" last="Restrepo">Silvia Restrepo</name><name sortKey="Rodriguez, Maria Juliana" sort="Rodriguez, Maria Juliana" uniqKey="Rodriguez M" first="María-Juliana" last="Rodríguez">María-Juliana Rodríguez</name><name sortKey="Valdes, Ivan" sort="Valdes, Ivan" uniqKey="Valdes I" first="Iván" last="Valdés">Iván Valdés</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000877 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000877 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30042788
|texte= A Genome-Scale Metabolic Reconstruction of Phytophthora infestans With the Integration of Transcriptional Data Reveals the Key Metabolic Patterns Involved in the Interaction of Its Host.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30042788" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |